Adverse Effect of Prolonged Formalin Fixation on DNA Histograms in Paraffin-Embedded Tissue.
نویسندگان
چکیده
منابع مشابه
DNA extraction from formalin-fixed, paraffin-embedded tissue.
Several DNA extraction methods have been used for formalin-fixed, paraffin-embedded tissues, with variable results being reported regarding the suitability of DNA obtained from such sources to serve as template in polymerase chain reaction (PCR)-based genetic analyses. We present a method routinely used for archival material in our laboratory that reliably yields DNA of sufficient quality for P...
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BACKGROUND Isolating amplifiable RNA from formalin-fixed, paraffin-embedded (FFPE) tissues is more difficult than isolating DNA because of RNases, chemical modification of the RNA, and cross-linking of nucleic acids and proteins. Tissues containing infectious disease agents that require biosafety level (BSL)-3 and -4 necessitate fixation times of 21 and 30 days, respectively. OBJECTIVE To imp...
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Biofluid detectable biomarkers that originate at the site of diseased tissues would be advantageous, in that, they may provide mechanistic information concerning the manifestation and progression of the disease. Unfortunately, tissue biopsies are precious samples that can generally be acquired in small amounts due to the invasive nature of the sample collection. One of the foundations of pathol...
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Title of dissertation: PROTEOME ANALYSIS OF FORMALIN-FIXED AND PARAFFINEMBEDDED TISSUE Tong Guo, Doctor of Philosophy, 2008 Dissertation directed by: Professor Cheng S. Lee Department of Chemistry and Biochemistry Because of the long history of the use of formalin as the standard fixative for tissue processing in histopathology, these archival formalin-fixed and paraffinembedded (FFPE) tissues ...
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ژورنال
عنوان ژورنال: ACTA HISTOCHEMICA ET CYTOCHEMICA
سال: 1998
ISSN: 0044-5991,1347-5800
DOI: 10.1267/ahc.31.171